Podoplanin and Tumor Inhibition

  • Ochoa-Alvarez, J.A.*, Krishnan, H.*, Shen, Y., Acharya, N.K., Han, M., McNulty, D.E., Hasegawa, H., Hyodo, T., Senga, T., Geng, J.-G., Kosciuk, M., Shin, S.S., Goydos, J.S., Temiakov, D., Nagele, R.G., and Goldberg, G.S. (2012) Plant lectin can target receptors containing sialic acid, exemplified by podoplanin, to inhibit transformed cell growth and migration. PLoS One 7, e41845. (* equal contribution) PMID: 22844530.
  • Krishnan, H., Ochoa-Alvarez, J.A., Shen, Y., Nevel, E., Lakshminarayanan, M., Williams, M.C., Ramirez, M.I., Miller, W.T., and Goldberg, G.S. (2013) Serines in the intracellular tail of podoplanin (PDPN) regulate cell motility. Journal of Biological Chemistry 288, 12215-12221. (published as “report” – reserved for “topics of exceptional novelty, significance and broad interest …. within the top 5 percent of all articles published in the journal”) PMID: 23530051.
  • Kolar, K., Freitas-Andrade, M., Bechberger, J.F., Krishnan, H., Goldberg, G.S., Naus, C.C., and Sin, W.C. (2015) Podoplanin: a marker of reactive gliosis in gliomas and brain injury. Journal of Neuropathology and Experimental Neurology 74, 64-74. (Highlighted in issue cover art). PMID: 25470350.
  • Ochoa-Alvarez, J.A., Krishnan, H., Pastorino, J.G., Nevel, E.M., Kephart, D., Lee, J.J., Retzbach, E.P., Shen, Y., Fatahzadeh, M., Baredes, S., Kalyoussef, E., Honma, M., Adelson, M.E., Kaneko, M.K., Kato, Y, Young, M.A., Deluca-Rapone, L., Shienbaum, A.J., Yin, K., Jensen, L.D., and Goldberg, G.S. (2015) Antibody and lectin target podoplanin to inhibit oral squamous carcinoma cell migration and viability by distinct mechanisms. Oncotarget 6, 9045- 90604. (highlighted in issue cover art and interviews with outstanding authors – www.youtube.com/watch?v=KWlWWA-FdjE-). PMID: 25826087.
  • Krishnan, H., Retzbach, E.P., Ramirez, M.I., Liu, T., Li, H., Miller, W.T., Goldberg, G.S. (2015) PKA and CDK5 can phosphorylate specific serines on the intracellular domain of podoplanin (PDPN) to inhibit cell motility. Experimental Cell Research 335, 115-122. PMID: 25959509.
  • Hamilton, K.L., Sheehan, S.A., Retzbach, E.P., Timmerman, C.A., Gianneschi, G.B., Tempera, P.J., Balachandran, P., and Goldberg, G.S. (2021) Effects of Maackia amurensis seed lectin (MASL) on oral squamous cell carcinoma (OSCC) gene expression and transcriptional signaling pathways. Journal of Cancer Research and Clinical Oncology. 147, 445–457. PMID: 33205348.
  • Retzbach, E.P., Sheehan, S.A., Krishnan, H., Zheng, H., Zhao, C., and Goldberg, G.S. (2022) Independent effects of Src kinase and podoplanin on anchorage independent cell growth and migration. Molecular Carcinogenesis. 61, 677-689. PMID: 35472679.

Contact Normalization and Tumor Migration

  • Goldberg, G.S., Alexander, D.B., Pellicena, P., Zhang, Z.-Y., Tsuda, H., and Miller, W.T. (2003) Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells. Journal of Biological Chemistry 278, 46533-46540. (highlighted in issue cover illustration) PMID: 12972425.
  • Alexander, D.B., Ichikawa, H., Bechberger, J.F., Valiunas, V., Ohki, M., Naus, C.C.G., .T., and Goldberg, G.S. (2004) Normal cells control the growth of neighboring transformed cells independent of gap junctional communication and Src activity. Cancer Research 64, 1347-1358. PMID: 14973064..
  • Valiunas, V., Bechberger, J.F., Naus, C.C.G., Brink, P.R., and Goldberg, G.S. (2005) Nontransformed cells can normalize gap junctional communication with transformed cells. Biochemical and Biophysical Research Communications 333, 174-179. PMID: 15936725.
  • Shen, Y., Jia Z., Nagele R.G., Ichikawa H., and Goldberg G.S. (2006) Src utilizes Cas to suppress Fhl1 in order to promote nonanchored growth and migration of tumor cells. Cancer Research 66, 1543-1552. PMID: 16452211.
  • Patwardhan, P., Shen, Y., Goldberg, G.S., and Miller, W.T. (2006) Individual Cas phosphorylation sites are dispensable for processive phosphorylation by Src and cellular transformation. Journal of Biological Chemistry 281, 20689-20697. PMID: 16707485.
  • Shen, Y., Khusial, P.R., Li, X., Ichikawa, H., Moreno, A.P., and Goldberg, G.S. (2007) Src utilizes Cas to block gap junctional communication mediated by connexin43. Journal of Biological Chemistry 282, 18914-18921. PMID: 17488714.
  • Li, X., Jia, Z., Shen, Y., Ichikawa, H., Jarvik, J., Nagele, R.J., and Goldberg, G.S. (2008) Coordinate suppression of Sdpr and Fhl1 expression in tumors of the breast, kidney, and prostate. Cancer Science 99, 1326-1333. PMID: 18422756.
  • Li, X., Shen, Y., Ichikawa, H., Antes, T., and Goldberg, G.S. (2009) Regulation of miRNA expression by Src and contact normalization: effects on nonanchored cell growth and migration. Oncogene 28, 4272–4283. PMID: 19767772.
  • Shen, Y., Chen, C.-S., Ichikawa, H., and Goldberg, G.S. (2010) Src induces Pdpn expression to promote cell migration. Journal of Biological Chemistry 285, 9649-9656. PMID: 20123990.
  • Funasaka, K., Ito, S., Hasegawa, H., Goldberg, G.S., Hirooka, Y., Goto, H., Hamaguchi, M., Senga, T. (2010) Cas utilizes Nck2 to activate Cdc42 and regulate cell polarization during cell migration in response to wound healing. FEBS Journal 277, 3502-3513. PMID: 20637038.
  • Khusial, P.R., Vadla, B., Krishnan, H., Ramlall, T.F., Shen, Y., Ichikawa, H., Geng, J.-G., and Goldberg, G.S. (2010) Src activates Abl to augment Robo1 expression in order to promote tumor cell migration. Oncotarget 4, 198-209. (highlighted in news section and issue cover illustration) PMID: 21301049

Cell To Cell Communication

  • Gibson, D.F., Hossain, M.Z., Goldberg, G.S., Acevedo, P., and Bertram, J.S. (1994) The mitogenic effects of transforming growth factors b1 and b2 in C3H/10T1/2 cells occur in the presence of enhanced gap junctional communication. Cell Growth and Differentiation 5, 687- 696. PMID: 8086343.
  • Goldberg, G.S., Martyn, K.D., and Lau, A.F. (1994) A connexin43 antisense vector reduces the ability of normal cells to inhibit the foci formation of transformed cells. Molecular Carcinogenesis 11, 106-114. PMID: 7916992.
  • Goldberg, G.S., Moreno, A.P., Bechberger, J.F., Hearn, S., Shivers, R.R., MacPhee, D.J., Zhang, Y.-C., and Naus, C.C.G. (1996) Evidence that disruption of connexon particle arrangements in gap junction plaques is associated with inhibition of gap junctional communication by a glycyrrhetinic acid derivative. Experimental Cell Research 222, 48-53. PMID: 8549672.
  • Orlando-Mathur, C.E., Bechberger, J.F., Goldberg, G.S., Naus, C.C.G., Kidder, G.M., and Kennedy, T.G. (1996) Rat endometrial stromal cells express the gap junction genes connexin26 and 43 and form functional gap junctions during in vitro decidualization. Biology of Reproduction 54, 905-913. PMID: 8924512.
  • Li, H., Liu, T.-F., Lazrak, A., Peracchia, C., Goldberg, G.S., Lampe, P.D., and Johnson, R. (1996) Properties and regulation of gap junctional hemichannels in the plasma membranes of cultured cells. Journal of Cell Biology 134, 1019-1030. PMID: 8769424.
  • Gibson, D.F.C., Bikle, D.D., Harris, J., and Goldberg, G.S. (1997) The expression of the gap junctional protein Cx43 is restricted to proliferating and non-differentiated normal and transformed keratinocytes. Experimental Dermatology 6, 167-174. PMID: 9293388.
  • Goldberg, G.S., Lampe, P.D., Sheedy, D., Stewart, C.C., Nicholson, B.J., and Naus, C.C.G. (1998) Direct identification and analysis of transjunctional ADP from Cx43 transfected C6 glioma cells. Experimental Cell Research 239, 82-92. PMID: 9511727.
  • Goldberg, G.S., Lampe, P.D., and Nicholson, B.J. (1999) Selective transfer of endogenous metabolites through gap junctions composed of different connexins. Nature Cell Biology 1, 457-459. PMID: 10559992.
  • Goldberg, G.S., Bechberger, J.F., Tajima, Y., Merritt, M., Omori, Y., Gawinowicz, M.A., Narayanan, R., Tan, Y., Sanai, Y., Yamasaki, H., Naus, C.C.G., Tsuda, H., and Nicholson, B.J. (2000) Connexin43 suppresses MFG-E8 while inducing contact growth inhibition of glioma cells. Cancer Research 60, 6018-6026. PMID: 11085522.
  • Goldberg, G.S., Jin, Z., Ichikawa, H., Naito, A., Ohki, M., El-Deiry, W.S., and Tsuda, H. (2001) Global effects of anchorage on gene expression during mammary carcinoma cell growth reveal role of tumor necrosis factor-related apoptosis ligand in anoikis. Cancer Research 61, 1334-1337.
  • Goldberg, G.S., Moreno, A.P., and Lampe, P.D. (2002) Gap junctions between cells expressing connexin 43 or 32 show inverse permselectivity to adenosine and ATP. Journal of Biological Chemistry 277, 36725-36730. PMID: 12119284.
  • Sheehan, S.A., Retzbach, E.P., Shen, Y., Krishnan, H., and Goldberg, G.S. (2022) Heterocellular N-cadherin junctions enable nontransformed cells to inhibit the growth of adjacent transformed cells. Cell Communication and Signaling. 20, 1-17. PMID: 35177067.

Books, Monographs and Chapters

Books & Monographs

  • Cancer Chemotherapy: Basic Science to the Clinic (2020) Goldberg, G.S. and Airley. R. Goldberg G.S. (Ed) Wiley-Blackwell, John Wiley and Sons, Ltd. ISBN-13: 978-1118963852.
  • Goldberg, G.S. and Martin-Villar, E. (2022) Molecular Mechanisms of Tumor Development and Progression – A Themed Honorary Issue to Prof. Miguel Quintanilla. Cells.
  • Fan, S., Parente-Arias, P., Goldberg, G.S., and Ferrone, S. (2022) Immunotherapy and Immune Regulation of Head and Neck Cancer. Frontiers in Oncology. Lausanne: Frontiers Media SA. doi: 10.3389/978-2-83250-861-9


  • Goldberg, G.S. and Moreno, A.P. (1998) Inhibition of connexin43 junctional conductance by 2,3-butanedione monoxime (BDM). In: Werner, R. (Ed.) Gap Junctions. pp. 210-214. Amsterdam: IOS Press.
  • Goldberg, G.S. and Lampe, P.D. (2001) Capture of endogenous transjunctional metabolites. In: Bruzzone R. and Giaume C. (Eds.) Methods in Molecular Biology; v. 154: Connexin Channels Methods and Protocols. pp. 329-340.Totowa NJ: Humana Press Inc. PMID: 11218656.
  • Naus, C.C., Goldberg, G.S., and Sin, W.C. (2005) Connexins in growth control and cancer. In: Winterhager, E. (Eds.) Gap junctions in development and disease. pp. 253-265. New York: Spinger-Verlag.
  • Goldberg, G.S. (2008) Contact Normalization. In: Schwab, M.E. (Eds.) Encyclopedia of Cancer. p. 750. Heidelberg: Spinger-Verlag.
  • Alvarez, J.A.O., George, C., Krishnan, H., Wu, X., and Goldberg, G.S. (2011) Contact Normalization: mechanisms and pathways to biomarkers and chemotherapeutic targets. In: Adams, J. (Ed.) Extracellular and Intracellular Signaling. pp. 105-115. Cambridge: Royal Society of Chemistry.
  • Krishnan, H. and Goldberg, G.S. (2015) Contact normalization or escape from the matrix. In: Kandous, M. (Ed.) Intercellular communication and Cancer. pp. 297-342. Heidelberg: Springer-Verlag.
  • Hellmig, T.J., Brace, E.J., Greenspan, A., Laugier, C., Aradhya, A., Basu, S., Parikh, S., Jachimowska, K., Wu, X., Shen, Y., Hamilton, K.L., and Goldberg, G.S. (2023) Amur maackia (Maackia amurensis) seed lectin history and potential effect on cancer progression, inflammation, and viral infection. In: Rajendram, R., Preedy, V. and Patel, V. (Ed.) Ancient and Traditional Foods, Plants, Herbs and Spices used in Cancer. pp. 89-101. Oxfordshire: Taylor and Francis.